Microfocused ultrasound (MFU) is a non-invasive treatment modality for skin tightening that induces multiple tiny thermal injury zones at focused areas resulting in neocollagenesis and skin lifting. This paper presents a 38-year-old female treated with MFU in the forehead and periorbital area, showing significant improvement in glabellar wrinkles, eyebrow lift, and skin regeneration. The pain during the MFU treatment was minimal, and the effects of the improvement in glabellar lines were maintained for eight weeks. The results suggest that MFU could be an effective treatment option for glabellar lines.