As the key part in the reinforced concrete column-steel beam (RCS) frame, the beam-column joints are usually subjected the axial force, shear force and bending moment under seismic actions. With the aim to study the seismic behavior of RCS joints with and without RC slab, the quasi-static cyclic tests results, including hysteretic curves, slab crack development, failure mode, strain distributions, etc. were discussed in detail. It is shown that the composite action between steel beam and RC slab can significantly enhance the initial stiffness and loading capacity, but lead to a changing of the failure mode from beam flexural failure to the joint shear failure. Based on the analysis of shear failure mechanism, the calculation formula accounting for the influence of RC slab was proposed to estimate shear strength of RCS joint. In addition, the finite element model (FEM) was developed by ABAQUS and a series of parametric analysis model with RC slab was conducted to investigate the influence of the face plates thickness, slab reinforcement diameter, beam web strength and inner concrete strength on the shear strength of joints. Finally, the proposed formula in this paper is verified by the experiment and FEM parametric analysis results.