This study aimed to improve the physicochemical properties of chitosan (CS) film by riboflavin (RF)-inducedcross-linking under ultraviolet (UV) light for different times (0, 2, 4, 6 h). It was found that the water vapor resistance, tensilestrength, optical property, and thermal stability of the film were significantly enhanced under RF-induced photo-crosslinking,especially when illuminated for 6 h. Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) verified themolecular bonding of CS and RF, as well as scanning electron microscope (SEM) showed a tighter and more uniform filmstructure was formed through UV crosslinking. Thus, the CS-RF films have the potential to replace petrochemical materialsas a novel type of environmentally friendly packaging material.