Objectives: This study aims to compare the push-out bond strength of fiber posts luted with self-adhesive resin cement with various pretreatments. Materials and Methods: Twenty-one single-rooted human premolar teeth were treated endodontically using a Ni-Ti rotary file and obturated with gutta-percha points and a sealer. The post room was immediately prepared to a depth of 12 mm with a post drill, while the teeth were divided into three groups: 1) no pretreatment (control); 2) pretreatment with 17% ethyl - enediaminetetraacetic acid (PE), and 3) pretreatment with 32% phosphoric acid (PP). Self-adhesive resin cement (G-CEM LinkAce) was applied directly to the post room. After application, a fiber post (LuxaPost) was immediately seated and lightcured for 40 s. The specimens were transversally sectioned to measure the regional push-out bond strengths. Results: The push-out bond strength was highest for all groups in the coronal parts and lowest in the apical parts (p<0.05). No significant differences according to the pretreatment were observed in the coronal part and middle parts. However, in the apical part, a significant difference was observed between the control and PE groups and between the control and PP groups (p < 0.05). Conclusion: Considering the limitations of this study, pretreatment with EDTA or phosphoric acid did not improve the push-out bond strength of fiber posts cemented with self-adhesive resin cement.