目的: 本研究旨在研究从韩国天然产品柿蒂(Diospyros kaki Thumb)中提取的纯化柿子单宁在化妆品中的潜 在应用。此外,通过γ射线照射降低其分子量来评估柿子单宁的功效。方法: 首先通过热水提取法提取高分子 柿子单宁聚合物。此外,通过γ射线照射其聚合物的分子量降低,随后,进行分离和纯化。利用对γ射线照射的 Kaki-tannin进行 DPPH、ABTS+ 和羟基自由基清除活性测定。此外,在体外测量了柿子单宁对B16F10黑色素 瘤细胞中酪氨酸酶和黑色素合成的抑制作用。此外,蛋白质印迹法证实了黑素生成过程中酪氨酸酶、小眼相关 转录因子 (MITF)、酪氨酸酶相关蛋白 (TRP)-1和TRP-2的表达。结果: 经辐照的柿单宁表现出抗氧化作用以及抑 制酪氨酸酶活性和黑色素生成。这项研究的结果证实,酪氨酸酶、MITF 和 TRP-2的表达随着辐照柿单宁处理的 增加而降低。结论: 在本研究中,为了在化妆品中加入高分子柿子单宁,首先用γ射线照射以减少其分子量。经 研究发现,照射的柿子单宁具有抗氧化和美白作用,并有潜力用作化妆品原料。
Purpose: This study aimed to investigate the use of purified Kaki-tannin obtained from a Korean natural product, Diospyros kaki Thumb. In order to confirm the potential application of kaki-tannin to cosmetics, the efficacy was evaluated by kaki-tannin that molecular weight decreased by gamma irradiation. Methods: Kaki-tannin polymer was extracted by hydrothermal extraction from persimmon. The molecular weight of Kaki-tannin polymer reduced by gamma-ray irradiation, and then separated and purified. Further, the molecular weight of this polymer was reduced by gamma-ray irradiation; subsequently, it was separated and purified. Radical scavenging activities of this reduced molecular weight Kaki-tannin were examined by the 2,2′-diphenyl-1-picrylhydrazyl (DPPH) radical, 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) diammonium salt radical cation, and hydroxyl radical scavenging assays. Moreover, the inhibitory effects of Kaki-tannin on tyrosinase and melanin synthesis in B16F10 melanoma cells were measured in vitro. Furthermore, the expression of tyrosinase, microphthalmia-associated transcription factor (MITF), tyrosinase-related protein (TRP)-1, and TRP-2 during melanogenesis was confirmed by western blotting. Results: Irradiated Kaki-tannin exhibited an antioxidant effect as well as suppressed tyrosinase activity and melanogenesis. The results of this study confirmed that the expression of tyrosinase, MITF, and TRP-2 decreased as the treatment of irradiated Kaki-tannin increased. Conclusion: In the present study, to incorporate high molecular weight Kaki-tannin in cosmetics, it was first irradiated with gamma rays in order to reduce its weight. This study confirmed that Kaki-tannin irradiated by gamma-ray has antioxidant and whitening effects as well as the potential to be used as a cosmetic material.