目的: 本研究调查了功能性美容剂“NEOGEN V.BIOME LIPOSOME™”对人真皮成纤维细胞 (HDFs)的抗皱效果。 这是一种采用专利技术形式 含有维生素 C 活性成分的脂质体。方法: “NEOGEN V.BIOME LIPOSOME™”的细胞 毒性通过水溶性四唑盐(WST-1)测定法进行测定。通过定量实时PCR(qRT-PCR) 测定法测量胶原蛋白 (COL1A1 ) 和乙酰透明质酸合酶 (HAS2 ) 的mRNA 表达。结果: “NEOGEN V.BIOME LIPOSOME™”对HDFs细胞生存率测定 结果,在浓度低于0.25%时没有细胞毒性。与阴性对照组相比,COL1A1 和HAS2 mRNA的表达呈浓度依赖性增 加。结论: “NEOGEN V.BIOME LIPOSOME™”通过促进 HDFs细胞中 COL1A1 和HAS2 mRNA的表达,有助于增 加胶原蛋白和透明质酸合酶的产生;因此,有望作为抗皱化妆品原料发挥作用。
Purpose: This study investigated functional cosmetic agent for anti-wrinkle effects of 'NEOGEN V.BIOME LIPOSOME™', which is a liposome in the form of a proprietary technology including a vitamin C active ingredient, on human dermal fibroblasts (HDFs). Methods: The cytotoxicity of 'NEOGEN V.BIOME LIPOSOME™' was measured by performing a water-soluble tetrazolium salt (WST-1) assay. The relative mRNA expression of collagen (COL1A1 ) and hyaluronan synthase (HAS2 ) was measured by quantitative real-time PCR (qRT-PCR) assay. Results: HDF cell viability of 'NEOGEN V.BIOME LIPOSOME™' was no cytotoxic effect at concentrations below 0.25%. The expression of COL1A1 and HAS2 mRNA was increased in a concentration-dependent manner compared with the negative control group. Conclusion: “NEOGEN V.BIOME LIPOSOME™“ helps to increase collagen and hyaluronan synthase production by promoting COL1A1 and HAS2 mRNA expression in HDFs; therefore, it is expected to function as an anti-wrinkle cosmetic material.