目的: 本研究评估了发芽和未发芽燕麦(Avena sativa )谷物的抗氧化和抗炎能力,以开发新的化妆品原料。 方法:将一些局部燕麦粒冷冻干燥(冷冻干燥),用95%乙醇提取,得到发芽的燕麦,再经干燥得到未发芽 的燕麦提取物。燕麦粒在20℃下发芽5天,并按照上述相同步骤提取。随后,将提取物应用于培养的细胞,以 确定其抗氧化,抗炎,抗黑色素生成和抗衰老作用。结果: 与发芽燕麦相比,发芽燕麦提取物的1,1-二苯基-2- 吡啶-2-肼基(DPPH)自由基清除活性和一氧化氮(NO)生成抑制能力分别提高了112%和43%。未发芽。 用脂多糖(LPS)刺激的RAW264.7细胞显示,用发芽的燕麦提取物处理可分别下调炎症因子(如环氧合酶 2,白介素1β和白介素6)的表达,分别下调35%,17.5%和22%。这种增强的抗炎作用可能是由于发芽期间 avenanthramide增加所致。结论: 发芽的燕麦籽提取物具有增强的抗氧化和抗炎特性。因此,发芽燕麦粒的提 取物可以成为化妆品工业的良好原料来源。
Purpose: This study evaluated the antioxidant and anti-inflammatory ability of germinated and non-germinated oat (Avena sativa ) grain for developing a new cosmetic raw material. Methods: Some local oat grains were freeze-dried (cryodesiccated) and extracted using 95% ethanol to obtain a germinated oat, and the non-germinated oat extract was obtained by redrying. The oat grains were germinated at 20℃ for 5 days and extracted using the same procedure as described above. Subsequently, the extracts were applied to cultured cells to determine their antioxidant, anti-inflammatory, anti-melanogenic, and anti-aging effects. Results: For the extract of germinated oat, 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity, and nitric oxide (NO) generation inhibition ability was increased to 112% and 43%, respectively, compared with the non-germinated. The RAW264.7 cells stimulated with lipopolysaccharides (LPS) revealed that treatment with the germinated oat extract could downregulate the expression of inflammatory factors, such as cyclooxygenase 2, interleukin 1beta, and interleukin 6 by 35%, 17.5%, and 22%, respectively. This enhanced anti-inflammatory effect could be as a result of increased avenanthramide during the germination period. Conclusion: Germinated oat grain extract showed an enhanced ability of antioxidant and anti-inflammatory properties. For this reason, the extract of germinated oat grain can be a good source of raw material for cosmetic industry.