In this study, We investigated not only the population densities of but also their population diversities by 16S rDNA analysis from the lactic acid bacteria in the spring water from august 2015 The results were as follows; Population densities of aerobic heterotrophic and oligotrophic bacteria by plate counting method were in the range of 1.61 ± 0.30 × 102 ~ 5.02 ± 1.45 × 102 cfu/ml and from 2.59 ± 0.34 × 102 ~ 7.26 ± 1.54 × 102 cfu/ml during sampling periods, respectively. We selected eight strains and identified by using 16S rDNA analysis. As a result, they were identified as Brevundimonas lenta. Pseudomonas nitroreducens, Pseudomonas putida, Sphingomonas aromaticivorans, Acidovorax delafieldii, Pseudomonas chlororaphis.