Pilots' behavior directly affects aviation safety. Exploring pilots' behavior can help to decode, simulate, and predict pilots' behavioral decisions. To examine the relationship between pilots' behavior and brain activity, this study used a flight simulator for the experiment and noninvasive functional near-infrared spectroscopy (fNIRS) for measurement and analyzed the change of oxyhemoglobin concentration in 25 pilot cadets with different turning behavior. The difference analysis between left-turning and right-turning was carried out based on this database. Results suggest that pilots' different turning behavior is related to the frontopolar area. The results of this study will contribute to helping people better understand the neuroscience mechanism of pilots ‘ behavior, and provide physiological reference values for improving pilots' behavior training.