Digital CRISPR-based quantification of HIV-1
- Resource Type
- Conference
- Authors
- Nouri, Reza; Jiang, Yuqian; Politza, Anthony J.; Lian, Xiaojun Lance; Guan, Weihua
- Source
- 2022 IEEE Sensors Sensors, 2022 IEEE. :1-4 Oct, 2022
- Subject
- Engineering Profession
General Topics for Engineers
Image resolution
RNA
Fluorescence
Sensor systems
Plasmas
Sensors
Testing
HIV-1
Digital assay
Cas13a
CRISPR
Nucleic acid testing
- Language
- ISSN
- 2168-9229
Here, we demonstrate a membrane-based digital CRISPR-Cas13a system for amplification-free absolute quantification of viral particles of HIV-1. We established a stamping technique to digitalize the Cas13 assay inside a commercial track-etched polycarbonate (PCTE) membrane. We evaluated the performance of our system by quantifying the synthetic HIV-1 RNA, where a limit of detection of 100 aM was achieved in 30 min reaction. Absolute quantification of the viral particles HIV-1 in plasma background using our system confirmed that our system can quantify spiked samples.