With the trend of miniaturization of advanced packaging technology, the number of Cu 6 Sn 5 intermetallic compound (IMC) grains is decreasing and then resulting anisotropic properties in the solder joints. Therefore, how to control the orientation of Cu 6 Sn 5 grains in micro solder joints has become a key issue for the reliability of 3D packaging. (001) and (011) single crystal Cu and polycrystalline Cu were specially designed as substrates to characterize the orientation evolution of Cu 6 Sn 5 grains reflowing by imposing temperature gradient (TG). There exists jagged interface in Cu/Sn/Cu and (011)Cu/Sn/Cu solder joints at the cold end between Cu 6 Sn 5 and solder comparing with the flat interface of (001)Cu/Sn/Cu solder joint. Rapid and directional growth of Cu 6 Sn 5 grains at the cold end with preferred orientation is observed on both single Cu substrates by imposing TG, which is completely distinct from the random orientation and scallop-type Cu 6 Sn 5 grains without TG. And the Cu 6 Sn 5 grains grow along the c-axis lying on the (001)Cu, i.e., the directions of Cu 6 Sn 5 are parallel to the interface between Cu and IMC. And the $ < 11\bar 20 > $ directions of Cu 6 Sn 5 are parallel to RD, which also is the direction of the temperature gradient, i.e., ${\left\{ {11\bar 20} \right\}_{{\eta }}}$ //{001} Cu . The Cu 6 Sn 5 grains in the (011)Cu/Sn/Cu solder joint at the cold end presents an about 30° angle with the Cu substrate. And there exists an intersecting angle about 90° between the two dominant directions. The perpendicular pairs prism-type Cu 6 Sn 5 grains grow along one direction on the (001)Cu surface due to the grain boundary migration driven by the large misorientation.