Evaluations of surgical biopsy, postmortem tissue specimens for the diagnosis and understanding of human disease are one of the critical components for biomedical studies. One of the severe brain tumors is the Glioma and histopathological tissue images can provide unique insights into identifying and grading disease stages. Actually, subtypes of Glioma is not specified so far. In this study, we tried to specify subtypes of LGG with the significant features related to nuclei. We employed CellProfiler [8] to implement our nuclei segmentation method. As a result, we discovered 3 subtypes of LGG using MORPHEUS [10] to create the heatmap.