3D Localization of Neurons in Bright-Field Histological Images
- Resource Type
- Conference
- Authors
- Stajduhar, Andrija; Lepage, Claude; Judas, Milos; Loncaric, Sven; Evans, Alan C.
- Source
- 2018 International Symposium ELMAR ELMAR, 2018 International Symposium. :75-78 Sep, 2018
- Subject
- Communication, Networking and Broadcast Technologies
Fields, Waves and Electromagnetics
Photonics and Electrooptics
Power, Energy and Industry Applications
Robotics and Control Systems
Signal Processing and Analysis
Neurons
Three-dimensional displays
Microscopy
Laplace equations
Splines (mathematics)
Two dimensional displays
High-resolution Imaging
Digital Microscopy
Immunohistochemistry
- Language
In this paper, we present a method for inferring the depth of neurons found in a bright-field microscopic image of a histological section of a human brain, digitized at high resolution in multiple planes along the z-axis. Individual neuron bodies are segmented and tracked throughout the depth of the whole image stack and placed at the appropriate z-level in the stack based on variations in image sharpness.