An embedded single-cell impedametric positioning tracker in microfluidic deformability assays
- Resource Type
- Conference
- Authors
- Ghassemi, Parham; Ren, Xiang; Strobl, Jeannine S.; Agah, Masoud
- Source
- 2017 19th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS) Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS), 2017 19th International Conference on. :1592-1595 Jun, 2017
- Subject
- Bioengineering
Components, Circuits, Devices and Systems
Photonics and Electrooptics
Power, Energy and Industry Applications
Robotics and Control Systems
Electrodes
Impedance
Velocity measurement
Impedance measurement
Microfluidics
Biomechanics
Loss measurement
Single-cell analysis
impedance spectroscopy
microfluidic assays
cancer cells
cell tracking
- Language
- ISSN
- 2167-0021
The paper reports the integration of bioimpedance sensing and cell deformability assays in a unique microfluidic channel for label-free and image-free analysis of single-cell biophysical (biomechanical) attributes. The all-transparent microfluidic chip consists of a constriction channel and an array of embedded indium-tin-oxide (ITO) electrodes through which cell position can be tracked with high resolution. Therefore, information regarding cell transit times and velocities during cell deformation can be readily assessed without the use of video microscopy. The deformability chip is also unique in that it separates the delivery and sensing channel thereby preventing cell clogging. The performance of the chip has been verified by single-cell analysis of breast cancer cells.