A micro device for impedance and mechanical characterization of biological cells
- Resource Type
- Conference
- Authors
- Chen, Jian; Zheng, Yi; Tan, Qingyuan; Zhang, Yan Liang; Li, Jason; To, Steve; Sun, Yu
- Source
- 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference Solid-State Sensors, Actuators and Microsystems Conference (TRANSDUCERS), 2011 16th International. :1192-1195 Jun, 2011
- Subject
- Bioengineering
Communication, Networking and Broadcast Technologies
Components, Circuits, Devices and Systems
Computing and Processing
Signal Processing and Analysis
Power, Energy and Industry Applications
Engineered Materials, Dielectrics and Plasmas
Fields, Waves and Electromagnetics
Robotics and Control Systems
Impedance
Integrated circuit modeling
Charge carrier processes
Impedance measurement
Data models
Cells (biology)
Young's modulus
Microfluidics
Cellular Biophysics
Single Cell Analysis
Impedance Spectroscopy
Micropipette Aspiration
- Language
- ISSN
- 2159-547X
2164-1641
This paper presents a microfluidic device for simultaneous electromechanical characterization of single cells. The device performs two types of cellular characterization (impedance spectroscopy and micropipette aspiration) on a single chip to enable cell electrical and mechanical characterization. To investigate the performance of the device design, electrical and mechanical properties of MC-3T3 osteoblast cells were measured. Based on electrical models, membrane capacitance of MC-3T3 cells was determined to be 3.39±1.23 pF and 2.99±0.82 pF at the aspiration pressure of 50 Pa and 100 Pa, respectively. Cytoplasm resistance values were 110.1±37.7 kΩ (50 Pa) and 145.2±44.3 kΩ (100 Pa). Aspiration length of cells was found to be 0.813±0.351 µm at 50 Pa and 1.771±0.623 µm at 100 Pa. Quantified Young's modulus values were 377±189 Pa at 50 Pa and 344±156 Pa at 100 Pa. Experimental results demonstrate the device's capability for characterizing both electrical and mechanical properties of single cells.