In this article, the fuzzy comprehensive analysis is applied to the evaluation and research of knowledge flow in supply chain by fuzzy mathematical theory. Firstly, this article designs an evaluation index system of knowledge flow in supply chain by means of questionnaire, and then it establishes the fuzzy comprehensive evaluation model of knowledge flow in supply chain, finally, it realizes grade comprehensive evaluation of knowledge flow in supply chain through the practical example. This article shows the fuzzy comprehensive analysis can be widely used in the evaluation of knowledge flow in supply chain owing to its strong interoperability and good effect.