A Single-molecule Manipulation Instrument Integrated with in Situ Nanopore Fabrication
- Resource Type
- Conference
- Authors
- Yin, Bohua; Fang, Shaoxi; Tang, Peng; Wang, Liang; Yin, Yajie; Wang, Yunjiao; Zhou, Shuo; Wang, Zuobin; Wang, Deqiang
- Source
- 2023 IEEE International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO) Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO), 2023 IEEE International Conference on. :422-425 Jul, 2023
- Subject
- Aerospace
Bioengineering
Components, Circuits, Devices and Systems
Computing and Processing
Engineered Materials, Dielectrics and Plasmas
Fields, Waves and Electromagnetics
General Topics for Engineers
Photonics and Electrooptics
Power, Energy and Industry Applications
Robotics and Control Systems
Signal Processing and Analysis
Fabrication
Proteins
Sequential analysis
Instruments
DNA
Genomics
Proteomics
single-molecule manipulation
solid-state nanopore
nanopipette
nanopore locating
λDNA
- Language
- ISSN
- 2694-510X
Nanopore sequencing is a powerful tool in the field of Genomics and Proteomics due to its long reads and identification of molecular modifications. However, there is still a challenge for locating a nanopore in manipulation-based sequencing with solid-state nanopore. In this paper, we developed an instrument for performing in situ nanopore fabrication and precise molecule manipulation in a seamless way. We successfully controlled a single λDNA molecule exiting a nanopore at a speed about 1 bp/s. The proof-of-utility of the instrument provides a strong support for the realization of solid-state nanopore-based sequencing.