This work presents the first multicentric, single arm, prospective study to evaluate the ability of MammoWave, a microwave imaging prototype, in breast lesions detection. This study was the first breast microwave imaging study during which both symptomatic and asymptomatic subjects were recruited. MammoWave output consists of a selection of microwave images’ features, determined prior to the beginning of the trials, to quantify images’ non-homogenous behavior. Our results on 382 breasts show a sensitivity of 82% using a statistical significance of p