Zinc (Zn) is an essential micronutrient for several physiological and biochemical processes. Changes in soil Zn levels can negatively affect plant physiology. Although the mechanism of Zn nutrition has been studied extensively in crops and model plants, there has been little research on steppe plants, particularly live in alkaline soils of arid and semiarid regions. Ceratoides arborescens is used in arid and semiarid regions as forage and ecological restoration germplasm, which is studied can enrich the mechanism of Zn nutrition. The plants were exposed to three different Zn treatments, Zn-deficient (-Zn 0 mM L−1), Zn-normal (Control, 0.015 mM L−1), and Zn-excess (+Zn, 0.15 mM L−1), for 3 weeks. Individual biomass, ion concentrations, photosynthetic system, and antioxidant characteristics were measured. High Zn supply significantly decreased plant biomass and induced chlorosis and growth defects and increased Zn concentration but decreased Fe and Ca concentrations, unlike in controls (p