Two different in vitro tests confirm the blocking activity of d-galactose lectins on the adhesion of Escherichia coli F4 to pig brush border receptors
- Resource Type
- article
- Authors
- Paolo Trevisi; Bevis Miller; Dilip Patel; Andrea Bolognesi; Massimo Bortolotti; Paolo Bosi
- Source
- Italian Journal of Animal Science, Vol 16, Iss 1, Pp 101-107 (2017)
- Subject
- Enterotoxigenic Escherichia coli F4
galactose lectin
Crotalaria juncea
intestinal adhesion
Animal culture
SF1-1100
- Language
- English
- ISSN
- 1828-051X
1828051X
The pathogenicity of Enterotoxigenic Escherichia coli (ETEC) expressing F4ac (K88) fimbrial adhesin and its adhesion to the pig jejunum surface is genetically controlled by the presence of a specific receptor glycoprotein on brush borders of the epithelium. We firstly screened the degree of inhibition obtained with ten galactose-recognising lectins – respectively from Abrus precatorius (APA-I); Coregonus maraena; Euphorbia characias; Crotalaria juncea; Sambucus ebulus (SEL1d); Sambucus nigra (SNAV); Coregonus peled; Momordica charantia (MCL); Trychosanthes kirilowii; Adenia racemosa –, by an ELISA test based on intestinal brush border sensitive for E. coli F4ac adhesion and ETEC F4ac fimbrial antigen. Three lectins significantly inhibited the fimbrial adhesion and the highest value was obtained by Crotalaria juncea lectin (p