Introduction and Objectives: Hepatic cells undergo different processes in response to the steatogenic input of MAFLD. Hepatic cell culture in steatogenic medium is a useful, reproducible tool intended to elucidate these pathogenic mechanisms. This study aimed to study cellular proliferation, death, and senescence in hepatocytes and hepatic stellate cells (HSC) using a model of steatosis in vitro. Materials and Methods: HepG2 hepatocytes were cultured in RPMI1640 (Control-Hep) and LX-2 HSC in DMEM (Control-LX2). Steatogenic media: either RPMI1640 or DMEM supplemented accordingly: mild steatosis (MS:50µM sodium oleate/sodium palmitate (OA/PA) at 2:1 ratio), severe steatosis (SS:500µM 2OA:1PA). HepG2 or LX-2 cells were preincubated for 24h at 37°C and 5% CO2, then incubated in MS or SS medium for up to 72h. Steatogenic medium was refreshed daily. Viability, mortality, proliferation, and senescence were analyzed. Assays are performed in triplicates. Data: Mean±SD. 2-way ANOVA followed by Tukey. P