Identification and application of a strong bidirectional acmN2p promoter from actinomycin D-producing streptomycetes
- Resource Type
- article
- Authors
- Sainan Li; Danfeng Tang; Xu Zhao; Manxiang Zhu; Xiangcheng Zhu; Yanwen Duan; Yong Huang
- Source
- Engineering Microbiology, Vol 4, Iss 1, Pp 100121- (2024)
- Subject
- Actinomycin D
Bidirectional promoter
Bohemamine
Streptomyces
Biotechnology
TP248.13-248.65
Microbiology
QR1-502
- Language
- English
- ISSN
- 2667-3703
Natural product biosynthesis is controlled at multiple levels. Characterization of naturally occurring promoters has facilitated the study of the synthetic biology of natural products. Herein, we report the discovery of two high-yield actinomycin D (ActD)-producing streptomycetes and the identification of a strong bidirectional acmN2p promoter from the ActD gene clusters and its application in heterologous expression of three core genes involved in the bacterial alkaloid bohemamine biosynthesis, providing a good example for identification of new promoters for synthetic biological applications.