Effect of Vitamin K2 Alone and in Combination with Calcium on the Bone Health Improvement and Mechanism in Zebrafish Model
- Resource Type
- article
- Authors
- Yi SUN; Hongzhi XIA; Kun NIU; Jiangbo LI; Yulei ZHU; Guyue LI; Zhongyan YIN
- Source
- Shipin gongye ke-ji, Vol 45, Iss 3, Pp 320-327 (2024)
- Subject
- vitamin k2
zebrafish
osteoporosis
mechanism
Food processing and manufacture
TP368-456
- Language
- Chinese
- ISSN
- 1002-0306
Objective: To investigate the bone health improvement effects and mechanism of vitamin K2 alone and in combination with calcium on osteoporotic zebrafish model. Methods: Healthy zebrafish with transgenic Tg line were used as experimental animals. The model of osteoporosis zebrafish was induced by prednisone. The fluorescence area and density of zebrafish osteoblasts were used as indicators to evaluate the bone development of zebrafish which were observed and photographed under microscope. Furthermore, the expression changes of related genes were detected by Real-time qPCR. Results: Vitamin K2 alone and in combination with D3 and calcium chloride could significantly improve the reduction of fluorescence area and fluorescence density of zebrafish osteoblasts induced by prednisolone in the experimental safe dose range of 5~20 μg/mL (P