目的 探讨慢性肾衰竭(CRF)大鼠肠道通透性及损伤的变化.方法 雄性SD大鼠20只分为CRF组(n=10)和对照组(n=10),采用5/6肾切除制备CRF大鼠模型,定期监测血生化至模型成功建立.术后第12周,大鼠禁食12 h后灌饲含有1 g乳果糖(L)和0.5 g甘露醇(M)的测试液4 ml,收集大鼠口服测试液后6 h内的全部尿液,采用高压液相色谱-示差法(HPLC-RID),检测尿液排泄率比值(L/M),评估大鼠肠黏膜通透性水平;HE染色观察各组大鼠小肠黏膜光镜下的形态(肠黏膜绒毛高度、肌层厚度、绒毛数量)并行组织学损伤评分.结果 CRF组大鼠尿L/M比值高于对照组(1.75±0.11比1.20±0.06,P<0.01);其肠黏膜绒毛高度、肌层厚度显著高于对照组(P<0.01),绒毛数量显著低于对照组(P<0.01).CRF组大鼠肠道病理组织学评分显著高于对照组(1.00±0.71比0,P<0.01).结论 CRF大鼠肠道通透性增加,并且伴有不同程度的肠道损伤.
Objective To observe the histopathologic injury of small intestine and intestinal permeability in chronic renal failure (CRF) rats. Methods Twenty male Sprague-Dawley rats were randomly assigned to CRF group (n=10) and control group (n=10). 5/6 nephrectomy was used to establish CRF rats, while sham operation for control. Blood biochemistry was regularly monitored until CRF model was successfully established. The model rats were fed with lactulose (L) and mannitol (M) through intragastric administration. Urine was collected after 6 hours, and the concentration of lactulose and mannitol in urine was measured using high pressure liquid chromatograph with refractive index detector (HPLC-RID), and the ratio of urinary excretion of L/M was calculated to evaluate intestinal permeability. Small intestinal mucosa were stained by hematoxylin-eosin (HE) and observed with light microscope (villus height, thickness of muscle layer and villus count), histological damage score was used to evaluate intestinal injury. Results The L/M ratio of CRF group was higher than that of control group (1.75±0.11 vs 1.20±0.06, P<0.01). The small intestinal mucosal villus height and thickness of muscle layer in CRF group were higher (P<0.01), and the number of villi was lower compared to control group (P<0.01). The score of histopathologic intestine damage of CRF group was higher than that of control group (1.00±0.71 vs 0, P<0.01). Conclusion The intestinal permeability of CRF rats is increased with varying degrees of intestinal damage.