Development and characterization of wheat-Aegilops kotschyi 1Uk(1A)substitution line with positive dough quality parameters
- Resource Type
- Academic Journal
- Authors
- JIANG Yun; WANG De-li; HAO Ming; ZHANG Jie; LIU Deng-cai
- Source
- 农业科学学报(英文版) / Journal of Integrative Agriculture. 22(4):999-1008
- Subject
- Aegilops kotschyi
common wheat
1Uk(1A)substitution line
HMW-GS
processing quality
- Language
- Chinese
- ISSN
- 2095-3119
Exploring novel high molecular weight glutenin subunits(HMW-GSs)from wild related species is a strategy to improve wheat processing quality.The objective of the present investigation was to identify the chromosomes of the wheat-alien introgression line N124,derived from the hybridization between Triticum aestivum with Aegilops kotschyi,and characterize the effects on quality-related traits.Fluorescence in situ hybridization karyotypes showed that N124 is a disomic 1Uk(1A)substitution line.Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)and reversed-phase high-performance liquid chromatography verified N124 expressed two HMW-GSs of the Ae.kotschyi parent.PacBio RNA sequencing and phylogenetic analysis confirmed that the two HMW-GSs were Ukx and Uky.Compared to the wheat parent,the substitution line had no obvious agronomic defects except fewer grains per spike but improved several major quality parameters.It can be served as a donor or bridge material for wheat quality improvement.