目的:探讨经过不同压力二氧化碳 (CO2) 气体控制后的卵巢癌细胞系A2780中基质金属蛋白酶-2、3 (MMP-2, 3) 和金属蛋白酶组织抑制剂-1、2 (TIMP-1, 2) 的相对表达量在压力控制后不同时间点的变化及意义.方法:选用卵巢癌细胞系A2780, 进行体外培养、细胞模型建立, 对照组于5%CO2孵箱中孵育3小时, 实验组通过体外模拟腹腔镜不同压力的CO2气腹环境, CO2压力分别控制在0 mmHg、7 mmHg、15 mmHg, 在CO2压力控制3小时后于0、12、24、48小时收集各组细胞.提取总RNA, 运用实时荧光定量聚合酶链式反应技术 (RT-qPCR) 检测各组样本中MMP-2、3和TIMP-1、2 mRNA相对表达量.结果:卵巢癌细胞经CO2压力控制后, 相同压力组各样本MMP-2 mRNA的相对表达量随压力控制后培养时间的延长而降低.经过CO2压力控制后卵巢癌细胞在相同时间点上MMP-2 mRNA的相对表达量随CO2压力的升高而降低.卵巢癌细胞经CO2压力控制后, 相同压力组各样本MMP-3 mRNA的相对表达量随压力控制后培养时间的延长而降低.经过CO2压力控制后卵巢癌细胞在相同时间点上MMP-3mRNA的相对表达量随CO2压力的升高而降低.对照组和实验组在不同时间点上和不同CO2压力情况下TIMP-1、2mRNA的相对表达量之间的两两比较, 差异无统计学意义 (P>0.05) .结论:模拟的CO2气腹环境可抑制卵巢癌细胞系A2780的侵袭能力, 并随时间延长及压力的增高MMP-2、3mRNA相对表达量降低.模拟的CO2气腹环境对卵巢癌细胞系A2780 TIMP-1、2mRNA的表达无明显影响.
Objective:To investigate the relative expression of matrix metalloproteinases-2, 3 (MMP-2, 3) and tissue inhibitors of metalloproteinases-1, 2 (Tissue Inhibitor of Metalloproteinase-1, 2, TIMP-1, 2) in ovarian cancer cell line A2780 under different pressures controlled by carbon dioxide (CO2) changes at different time points and their meaning.Methods:Cell model was established by culture and grouping of A2780 ovarian cancer cell line in vitro.By simulation of laparoscopic CO2pneumoperitoneum environment under different pressure in vitro the pressure of carbon dioxide is controlled at 0 mmHg, 7 mmHg and 15 mmHg respectirely, the cells were collected at 0, 12, 24 and 48 hours after the CO2pressure was controlled for 3 hours.Total RNA was extracted and RT-qPCR was performed to detect the relative expression of MMP-2, 3, TIMP-1 and 2 mRNA in each group.Results:After CO2pressure control, the relative expression of MMP-2 mRNA in each sample under the same pressure decreased with the duration of culture.After CO2pressure control, the relative expression of MMP-2 in ovarian cancer cells decreased with the increase of CO2pressure at the same time point.After CO2pressure control, the relative expression of MMP-3 mRNA in each sample underthe same pressure decreased with the culture time.After CO2pressure control, the relative expression of MMP-3 in ovarian cancer cells decreased with the increase of CO2pressure at the same time point.There was no significant difference in the relative expression of TIMP-1 and TIMP-2 mRNA between the control group and the experimental group at different time points orunder different CO2pressures (P>0.05).Conclusions:The simulated CO2pneumoperitoneum environment could inhibit the invasive ability of ovarian cancer cell line A2780.The relative expression of MMP-2 and MMP-3 decreased astime and pressure increasing.The simulated CO2pneumoperitoneum environment had no significant effect on the expression of A2780 TIMP-1 and 2 mRNA in ovarian cancer cell line A2780.