设计3个冷藏组(7、14、21 d)和2个对照组(未处理、常温21 d),通过厌氧发酵接种物的理化指标(pH值、VFA、TIC、NH4+-N)测定、菌落结构分析和厌氧发酵产甲烷能力测试,研究了4℃冷藏处理对厌氧发酵接种物活性的影响。结果表明:接种物在4℃冷藏过程中,代表产甲烷菌的优势条带随冷藏时间的延长出现了亮度下降,并有少量条带丢失的现象;其厌氧发酵产甲烷能力出现下降,前15 d的日产甲烷速率明显低于未处理组的;但冷藏处理能够减缓氨氮浓度的增高,有助于接种物的厌氧发酵活性的恢复。
In this study, the inoculum for anaerobic fermentation was stored under five different conditions formed by 3 cold (4 ℃) storage groups (7 d, 14 d, 21 d) and 2 control groups (untreated storage, room-temperature storage for 21 d), its physical and chemical properties (pH-value, VFA, TIC, and NH4+-N), colony structure and anaerobic methanogenesis ability were tested and analyzed, and the effect of cold storage at 4 ℃ on its activity was studied.The results showed that: during the cold storage of anaerobic fermentation inoculum at 4℃, the quantity and varieties of methanogens were declined , and their daily anaerobic methanogenesis rate at earlier 15-d stage was obviously lower than that in the untreated control group.However, the cold storage could reduce ammonia nitrogen concentration and contribute to the recovery of anaerobic fermentation activity of inocu-lum.