目的 探讨急性细菌和病毒感染患儿血清γ干扰素诱导蛋白10(IP-10)的水平变化及临床价值.方法 选取2013年1月至2016年4月湖南省人民医院住院的急性病毒感染患儿51例、急性细菌感染患儿52例为研究对象,对照组选取同期该院体检未发现感染疾病者51例.采用酶联免疫吸附法检测血浆IP-10水平.采用西门子BNⅡ特定蛋白分析仪测定血清C反应蛋白(CRP).结果 病毒感染组的IP-10水平明显高于细菌感染组和对照组(P<0.05).细菌感染组IP-10明显高于对照组(P<0.05).病毒感染组的CRP水平明显低于细菌感染组(P<0.05),但高于对照组(P<0.05).受试者工作特征曲线分析,IP-10和CRP在区分急性病原体感染患者和对照组人群的曲线下面积分别为0.688和0.873,灵敏度分别为35.0%、79.6%,特异度分别为96.1%、98.0%.IP-10与CRP联合检测的灵敏度和特异度分别提高到82.5% 和100.0%.结论 高水平血浆IP-10可能对患儿发生急性感染有一定的预测价值.IP-10和CRP联合检测提高急性病原体感染诊断效能.
Objective To observe the levels of interferon gamma-induced protein 10(IP-10)in children with acute viral and bacterial infection.Methods There were three groups:acute viral infection group(51 ca-ses),acute bacterial infection group(52 cases),and healthy control group(51 cases).Serum IP-10 levels were detected by enzyme linked immunosorbent assay(ELISA),and serum C reactive protein(CRP)were deter-mined with BNⅡ automatic protein analyzer.Results The level of serum IP-10 and CRP were significantly different in different groups(P<0.05).Compared with control group,the level of IP-10 and CRP were higher in acute viral infection group or in acute bacterial infection group(P<0.05).The level of IP-10 was higher in acute viral infection group than that in acute bacterial infection group(P>0.05).The level of CRP was higher in bacterial infection group than that in acute viral infection group(P<0.05).The IP-10 levels in viral Infec-tion patients were not correlated with the CRP levels.The area under curve(AUC),sensitivity and specificity of IP-10 and CRP were 0.688 and 0.873,35.0% and 79.6%,96.1% and 98.0%.When cut-off value of predic-tive probability was 0.713,sensitivity and specificity were increased to 82.5% and 100.0%.Conclusion Ser-um IP-10,CRP and predictive probability are valuable in the diagnosis of acute pathogen infection in children.