Watt-level all polarization-maintaining femtosecond fiber laser source at 1100 nm for multicolor two-photon fluorescence excitation of fluorescent proteins
- Resource Type
- Working Paper
- Authors
- Wen, Junpeng; Pilger, Christian; Wang, Wenlong; Erapaneedi, Raghu; Xiu, Hao; Fan, Yiheng; Hu, Xu; Huser, Thomas; Kiefer, Friedemann; Wei, Xiaoming; Yang, Zhongmin
- Source
- Subject
- Physics - Optics
Physics - Biological Physics
- Language
We demonstrate a compact watt-level all polarization-maintaining (PM) femtosecond fiber laser source at 1100 nm. The fiber laser source is seeded by an all PM fiber mode-locked laser employing a nonlinear amplifying loop mirror. The seed laser can generate stable pulses at a fundamental repetition rate of 40.71 MHz with a signal-to-noise rate of >100 dB and an integrated relative intensity noise of only ~0.061%. After two-stage external amplification and pulse compression, an output power of ~1.47 W (corresponding to a pulse energy of ~36.1 nJ) and a pulse duration of ~251 fs are obtained. The 1100 nm femtosecond fiber laser is then employed as the excitation light source for multicolor multi-photon fluorescence microscopy of Chinese hamster ovary (CHO) cells stably expressing red fluorescent proteins.