[Expressions of IL-18Ralpha and IL-18Rbeta on rat primary renal tubular epithelial cells]
- Resource Type
- Authors
- Sheng-hua, Du; Hua-feng, Liu; De-shen, Tang; Xiao-wen, Chen
- Source
- Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 21(3)
- Subject
- Male
Kidney Tubules
Gene Expression Regulation
Animals
Epithelial Cells
Female
RNA, Messenger
Interleukin-18 Receptor beta Subunit
Fibrosis
Immunohistochemistry
Interleukin-18 Receptor alpha Subunit
Rats
- Language
- ISSN
- 1007-8738
To detect the expressions of the IL-18Ralpha mRNA and IL-18Rbeta mRNA in primary rat renal tubular epithelial cells (RTECs).The culture of primary RTECs was performed by renal tubular segment sticking mass method. The cellular type was identified by immunocytochemical staining. The IL-18Ralpha mRNA and IL-18Rbeta mRNA expressions in RTECs were detected by RT-PCR.The result of immunocytochemical staining proved that the cultured cells were RTECs, IL-18Ralpha mRNA and IL-18Rbeta mRNA were detected in RTECs.IL-18Ralpha mRNA and IL-18Rbeta mRNA are expressed in RTECs, which provide the experimental basis for exploring IL-18R role in renal interstitial fibrosis.