Robust and sensitive detection of gene fusions using high-throughput SMART-Seq chemistry on the ICELL8 cx system
- Resource Type
- Authors
- Gandlur, Suvarna; Fish, Rachel; Young, Michael; Liu, Lyndon; Wei, Sherry; Swaminathan, Karthikeyan; Bostick, Magnolia; Farmer, Andrew; Ashworth, Roxann; Knudtson, Kevin
- Source
- J Biomol Tech
- Subject
- ComputingMethodologies_PATTERNRECOGNITION
Poster Abstracts
- Language
- English
Isolating single cells at high-throughput levels and obtaining their full-length transcript information has become critical to the scientific community to generate rich single-cell datasets. We automated SMART-Seq® chemistry on the ICELL8® cx Single-Cell System to address this need and show this workflow provides useful information that end-capture technologies cannot. We present performance data showing that capturing junction and spanning reads with this automated, full-length mRNA-seq method enables confident and robust identification of gene fusions in a breast cancer tumor cell line.