Epithelial morphogenesis is one of the crucial processes of tissue development, and is mediated by cell-cell communication. A key characteristic of epithelial cells is their polarization along the apical-basal axis. Loss of cell polarity often leads to neoplasia in metazoans, therefore several key components involved in apicobasal polarity formation have been identified to be tumour suppressors. However, it remains largely unknown how epithelial polarity and growth control are mutually coordinated. By using Drosophila melanogaster wing development and mammalian cell culture as model systems, this study aims to address mechanisms underlying apicobasal polarity and tissue development. In the Drosophila wing imaginal disc, via studying polarity determinant Scribble (Scrib), this study reveals that cell polarity is regulated through intercellular mechanism. Conditional knockdown of scrib in a stripe of cells in the wing imaginal disc results in loss of polarity in adjacent wild type cells, ultimately leading to neoplasia. Yorkie activity, an effector of the Hippo signalling pathway, is required for the expansion of loss of cell polarity. The data show that genetic interaction between the septate junction (SJ), Scribble and α-Catenin axis plays an important role in intercellular regulation of cell polarity. Next, this study demonstrates that Scribble physically interacts with α-catenin (α-Cat), and this interaction is conserved in Drosophila and mammals. The Scrib-α-Cat association helps stabilize localization of Scribble and regulates tissue homeostasis in Drosophila. The complex of Scrib and α-Cat regulates cell proliferation through the Hippo pathway in Drosophila and mammalian cells. In Drosophila, BMP/Dpp signalling is crucial for wing vein morphogenesis. In pupal wing, Dpp is expressed in the longitudinal veins (LVs) and is transported to the posterior crossvein (PCV) region to induce PCV morphogenesis. This study reveals that Scribble is required for PCV development. The data show that Scribble regulates BMP signalling by affecting the internalization of BMP type I receptor Thickveins (Tkv) in the PCV region. BMP signalling induces Scribble transcription, thus forming a feedback loop between Scrib and BMP signalling, which play a crucial role in PCV morphogenesis. Overall, this study reveals that Scribble plays a unique role in tissue growth in a non-autonomous manner through regulating apicobasal polarity at the tissue level. Conserved roles of interaction between Scribble and α-Cat in vertebrates and invertebrates provide important insights into tissue growth and homeostasis. Finally, Scribble contributes to pattern formation by forming a feedback loop with BMP signalling in tissue development. Taken together, this thesis work provides novel insights into how polarity determinant Scribble is utilized for tissue development through its interactions with multiple partners. Tiivistelmä ei saatavilla.