Precursor diversity and complexity of lineage relationships in the outer subventricular zone of the primate
- Resource Type
- Authors
- Sabina Pfister; C. Huissoud; Henry Kennedy; Colette Dehay; Elodie Gautier; Rodney J. Douglas; Dorothée Patti; Marielle Afanassieff; Angele Bellemin-Menard; Marion Betizeau; Veronique Cortay
- Source
- Neuron
Neuron, Elsevier, 2013, 80 (2), pp.442-457. ⟨10.1016/j.neuron.2013.09.032⟩
- Subject
- Lineage (genetic)
PAX6 Transcription Factor
Neurogenesis
Neuroscience(all)
[SDV]Life Sciences [q-bio]
Subventricular zone
[INFO] Computer Science [cs]
Macaque
03 medical and health sciences
Basal (phylogenetics)
0302 clinical medicine
Neural Stem Cells
Live cell imaging
Lateral Ventricles
biology.animal
medicine
Animals
Paired Box Transcription Factors
Cell Lineage
Primate
[INFO]Computer Science [cs]
Eye Proteins
Cells, Cultured
10194 Institute of Neuroinformatics
030304 developmental biology
Cerebral Cortex
Homeodomain Proteins
Regulation of gene expression
0303 health sciences
biology
General Neuroscience
Cell Cycle
Gene Expression Regulation, Developmental
2800 General Neuroscience
Anatomy
Cell biology
Repressor Proteins
[SDV] Life Sciences [q-bio]
Macaca fascicularis
Corticogenesis
medicine.anatomical_structure
570 Life sciences
T-Box Domain Proteins
030217 neurology & neurosurgery
- Language
- English
- ISSN
- 0896-6273
International audience; Long-term ex vivo live imaging combined with unbiased sampling of cycling precursors shows that macaque outer subventricular zone (OSVZ) includes four distinct basal radial glial (bRG) cell morpho-types, bearing apical and/or basal processes in addition to nonpolar intermediate progenitors (IPs). Each of the five precursor types exhibits extensive self-renewal and proliferative capacities as well as the ability to directly generate neurons, albeit with different frequencies. Cell-cycle parameters exhibited an unusual stage-specific regulation with short cell-cycle duration and increased rates of proliferative divisions during supragranular layer production at late corticogenesis. State transition analysis of an extensive clonal database reveals bidirectional transitions between OSVZ precursor types as well as stage-specific differences in their progeny and topology of the lineage relationships. These results explore rodent-primate differences and show that primate cortical neurons are generated through complex lineages by a mosaic of precursors, thereby providing an innovative framework for understanding specific features of primate corticogenesis.