Protocol to detect antigen-specific nasal-resident T cells in humans
- Resource Type
- Authors
- Anthony Tanoto TAN; Joey Ming Er LIM; Antonio Bertoletti
- Source
- STAR Protocols. 4:101995
- Subject
- General Immunology and Microbiology
General Neuroscience
General Biochemistry, Genetics and Molecular Biology
- Language
- ISSN
- 2666-1667
Specialized T cells are located in the nasal cavity and act as the first line of defense against respiratory viral infection. Here, we present a protocol for the detection and characterization of antigen-specific nasal-resident T cells. We detail steps for localized nasal swabbing to collect the nasal samples. We then describe IFN-γ ELISpot and an activation-induced marker assay to detect and characterize antigen-specific nasal-resident T cells. For complete details on the use and execution of this protocol, please refer to Lim et al. (2022).