Additional file 1: Fig. S1. Confirmation of CRISPR/cas9-edited AtSPT4-2 knockout mutants through sequencing and expression analysis of AtSPT4-2 overexpression lines through qRT–PCR. a The sequence with mutations in knockout mutant lines compared with the wild-type sequence. Arrows indicate the mutation sites. The deletion in the DNA sequences was designated with “-”. sgRNA, single guide RNA; PAM, protospacer adjacent motif. b Relative expression of AtSPT4-2 in OE7, OE10, and wild type (WT). Ten-day-old seedlings grown on MS medium were used to quantify the expression of AtSPT4-2 in transgenic plants along with wild type as a control using qRT–PCR. Ubiquitin was used as an internal reference gene. The values are the mean ± SD (n = 3 experiments). Table S1. List of overlapping genes related to salt stress between the KO vs WT-control, OE vs WT-control, KO vs WT-salt and OE vs WT-salt groups. Table S2. List of primers used in this study.