Additional file 6: Figure S4. The combination of SB-699551 and docetaxel increases the frequency of apoptotic tumor cells in vivo. (A) Histological sections of 3 tumors from each cohort were prepared and stained with H&E. The red arrows indicate fibrotic areas that are devoid of tumor cells. The green arrows identify cells undergoing apoptosis with a characteristic shrunken appearance and pyknotic nuclei. The top 3 rows were imaged at 200X magnification, whereas the bottom row was imaged at 400X. (B) Tumor sections were stained using the TUNEL assay and imaged under the 4′,6-diamidino-2-phenylindole (DAPI) channel (top row), the fluorescein isothiocyanate (FITC) channel (middle row) and merged (bottom row). Images were taken at 100X magnification. Scale bars represents 100 μm.