Additional file 1: Supplementary Fig. 1. Apoptosis analysis after SAW treatment. hBMECs were seeded in round slides for 48 h when firm barrier formed, then the slides were exposed on a SAW. The apoptosis of hBMECs was measured with Annexin V-AbFluor-647 Apoptosis Detection kit. Data are representative results of two individual experiments. Supplementary Table 1. The statistical analysis of Supplementary Fig. 1. Supplementary Fig. 2. Three-dimensionalimage of tight junction proteins. hBMECs were seeded in round slides for 48 h when firm barrier formed, then the slides were exposed on a SAW, which was same with Fig. 6. The z axis was selected for 10 μm, and 11 continuous pictures were acquired. The 3-D distribution of cell tight junction proteins was reconstituted and shown. Supplementary Fig. 3. A schematic diagram of brain region in 10-day-old zebrafish. The main brain regions include telencephalon, optic tectum, cerebellum and brainstem. The dotted lines only indicate the relative position of each brain region, and the area does not represent the full size of each region. Scale bar was 200 μm.