Expression of the nucleoside transporters hENT1 (SLC29) and hCNT1 (SLC28) in pediatric acute myeloid leukemia
- Resource Type
- Authors
- Adrian Christopher Jaramillo; Marçal Pastor-Anglada; Godefridus J. Peters; Jacqueline Cloos; Gertjan J.L. Kaspers; Richard Broekhuizen; Isabelle Hubeek; Gerrit Jansen
- Source
- Jaramillo, A C, Hubeek, I, Broekhuizen, R, Pastor-Anglada, M, Kaspers, G J L, Jansen, G, Cloos, J & Peters, G J 2020, ' Expression of the nucleoside transporters hENT1 (SLC29) and hCNT1 (SLC28) in pediatric acute myeloid leukemia ', Nucleosides, Nucleotides and Nucleic Acids, vol. 39, no. 10-12, pp. 1379-1388 . https://doi.org/10.1080/15257770.2020.1746803
Nucleosides, Nucleotides and Nucleic Acids, 39(10-12), 1379-1388. Taylor and Francis Ltd.
- Subject
- Male
Adolescent
010402 general chemistry
Equilibrative nucleoside transporter 1
01 natural sciences
Biochemistry
Equilibrative Nucleoside Transporter 1
Genetics
Humans
RNA, Messenger
Child
biology
010405 organic chemistry
Chemistry
Pediatric acute myeloid leukemia
Infant, Newborn
Infant
Membrane Transport Proteins
Equilibrative nucleoside transporter
Transporter
General Medicine
0104 chemical sciences
Gene Expression Regulation, Neoplastic
Leukemia, Myeloid, Acute
Child, Preschool
Cancer research
biology.protein
Molecular Medicine
Female
Nucleoside
- Language
- English
- ISSN
- 1525-7770
Cellular uptake of clinically important deoxynucleoside analogs is mediated by nucleoside transporters including the human equilibrative nucleoside transporter 1 (hENT1) and the concentrative nucleoside transporter-1 (hCNT1). These transporters are responsible for influx of cytarabine and reduced hENT1 expression is a major resistance mechanism in acute myeloid leukemia. We determined hENT1 and hCNT1 protein expression by immunocytochemistry in 50 diagnostic pediatric acute myeloid leukemia patient samples. All samples expressed hENT1 [9/43 (21%) low; 26/43 (60%) medium and 8/43 (19%) high] and hCNT1 [2/42 (5%) low; 35/42 (83%) medium and 5/42 (12%) high] at the cell membrane and cytoplasm. Statistical analysis showed a non-significant relationship between survival and transporter expression and in vitro drug sensitivity. In conclusion, the nucleoside transporters hENT1 and hCNT1 are broadly expressed in pediatric acute myeloid leukemia at diagnosis.