Additional file 2 of Siglec-15-induced autophagy promotes invasion and metastasis of human osteosarcoma cells by activating the epithelial–mesenchymal transition and Beclin-1/ATG14 pathway
- Resource Type
- Authors
- Zheng, Bingxin; Song, Keliang; Sun, Lingling; Gao, Yang; Qu, Yan; Ren, Chongmin; Yan, Peng; Chen, Wenfang; Guo, Wei; Zhou, Chuanli; Yue, Bin
- Source
- Subject
- respiratory system
- Language
Additional file 2: FigureS2. Effect of Siglec-15 expressionrecovery on migration, invasion and autophagy of osteosarcoma cells. (a) Westernblots were used to detect the expression of EMT and autophagy-related proteinsin shSiglec-15-KHOS cells recovering Siglec-15 with or without 3-MA treatment. (b) Cells after Siglec-15 expression recovery increased apunctate pattern of LC3-II fluorescence. shSiglec-15-KHOS cells were incubatedwith or without 3-MA. (c) Transwell assay was used to detect the invasion andmigration ability of shSiglec-15-KHOS cells with or without 3-MA treatment onthe basis of Siglec-15 expression recovery. These experiments were repeated 3times. Data are presented as the mean ± S.D. (**P < 0.01).