Expression of TNF-alpha in oral lichen planus treated with fluocinolone acetonide 0.1%
- Resource Type
- Authors
- Kittipong Dhanuthai; Kobkan Thongprasom; Mantharop Chaimusig; Ponlatham Chaiyarit; Wilairat Sarideechaigul
- Source
- Journal of Oral Pathology and Medicine. 35:161-166
- Subject
- Adult
Male
Cancer Research
Pathology
medicine.medical_specialty
Adolescent
medicine.drug_class
medicine.medical_treatment
Peripheral blood mononuclear cell
Pathology and Forensic Medicine
stomatognathic system
Fluocinolone acetonide
Biopsy
medicine
Humans
Glucocorticoids
Aged
Chemotherapy
biology
medicine.diagnostic_test
Tumor Necrosis Factor-alpha
business.industry
Middle Aged
medicine.disease
stomatognathic diseases
Fluocinolone Acetonide
Otorhinolaryngology
Carboxymethylcellulose Sodium
biology.protein
Periodontics
Corticosteroid
Female
Tumor necrosis factor alpha
Oral lichen planus
Oral Surgery
Antibody
business
Lichen Planus, Oral
medicine.drug
- Language
- ISSN
- 1600-0714
0904-2512
Background Oral lichen planus (OLP) is a common chronic inflammatory disease involving T cells-mediated immunity. Tumor necrosis factor (TNF)-alpha has been reported to be involved in the disease process. The purpose of this study was to investigate the effect of fluocinolone acetonide in orabase (FAO) 0.1% on the expression of TNF-alpha in patients with OLP. Methods Eighteen Thai patients with atrophic or erosive OLP were recruited. Biopsy specimens were taken before and 1 month after treatment with FAO 0.1% and sent for histopathologic examination where they were immunohistochemically stained with antibody to TNF-alpha. Twenty normal mucosa specimens were identically processed. Oral squamous cell carcinoma tissue was used as a positive control for TNF-alpha expression, whereas OLP sections without primary antibody were served as negative control. Results Sixteen of 18 cases (88.89%) of OLP exhibited positive staining for TNF-alpha. Most of the TNF-alpha was observed in the mononuclear cells. Ten cases (55.56%) of OLP demonstrated TNF-alpha expression in keratinocytes. The number of mononuclear cells positive for TNF-alpha before the treatment with FAO 0.1% in orabase was statistically higher than that after the treatment (P=0.000) and in the normal mucosa (P=0.000). Moreover, the number of mononuclear cells after treatment with FAO 0.1% in orabase was statistically lower than before the treatment (P=0.000). Conclusion Our study exhibited that TNF-alpha may be associated with the immunopathogenesis of OLP in Thai patients and FAO 0.1% had an effect on the reduction of TNF-alpha expression.