Additional file 2. Supplemental Figure 2: Increased cytokine and chemokine production from ERAP1-/- macrophages following NLRP3 inflammasome activation. Heat map depicting the top 12 upregulated and downregulated cytokines by ERAP1. Bone marrow-derived macrophages (BMDMs) from C57 WT and ERAP1-/- mice were primed with LPS (20 ng/mL) for 12 hours. Cells were then stimulated with nigericin (10 ug/mL) for an additional 20 hours. Supernatants were collected and analyzed used in a 23-plex multiplex assay using Bioplex.