Additional file 1 of Efficient production of a cyclic dipeptide (cyclo-TA) using heterologous expression system of filamentous fungus Aspergillus oryzae
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- Qi, Jianzhao; Han, Haiyan; Sui, Dan; Tan, Shengnan; Liu, Changli; Wang, Pengchao; Xie, Chunliang; Xia, Xuekui; Gao, Jin-ming; Liu, Chengwei
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Additional file 1. Supplementary experimental section. Fig. S1. Mycelial morphology of E. cristatum NWAFU-1. Fig. S2. Molecular network of the metabolic products from E. cristatum NWAFU-1. Fig. S3. Proposed biosynthetic gene clusters of echinulin and function analysis of each gene in the BGC. Fig. S4. Sequence similarity network analysis of CriC. Fig. S5. 1H-NMR spectrum of Cyclo-TA (CD3OD-d4, 500 MHz). Fig. S6. 13C-NMR spectrum of Cyclo-TA (CD3OD-d4, 125 MHz). Fig. S7. HSQC spectrum of Cyclo-TA (CD3OD-d4). Fig. S8. HMBC spectrum of Cyclo-TA (CD3OD-d4). Fig. S9. 1H-1H COSY spectrum of Cyclo-TA (CD3OD-d4). Fig. S10. NOESY spectrum of Cyclo-TA (CD3OD-d4). Fig. S11. HPLC traces of time-course biochemical assays for microsome containing CriC. Fig. S12. Substrate promiscuity analysis of CriC. Fig. S13. HPLC traces of AO-criC product under non-linear increasing concentration gradient substrate feeding. Fig. S14. Domain analysis and speculative reaction mechanism for CriC. Fig. S15. Genome mining-based CriC uncovered serval BGCs responsible for Cyclo-TA containing compounds. Table S1. Primers used for construction of expression plasmids. Table S2. NMR Data of Cyclo-TA in CD3OD-d4 (500 MHz for 1H NMR, 125 MHz for 13C NMR). Table S3. Percent identity matrix of CriC and its homologies in CriC group branch on the evolutionary tree.