Additional file 2: S1. Optimization of Extraction Processing for Bioactive Ingredients. Table S1. The Box-Behnken design experiment scheme. Table S2. ANOVA of quadratic model terms of three factor variables on responses. Table S3. Predicted and experimental values of the responses obtained under the optimal extraction conditions. Figure S1. Result of the single factor experiments. Figure S2. Response surface plots corresponding to the desirability function.