Genome-Wide Association Study of Muscle Glycogen in Jingxing Yellow Chicken
- Resource Type
- Authors
- Guiping Zhao; Huanxian Cui; Jie Wen; Huijuan Bi; Huanhuan Chu; Lu Liu; Xiaojing Liu; Jie Wang
- Source
- Genes
Volume 11
Issue 5
Genes, Vol 11, Iss 497, p 497 (2020)
- Subject
- 0301 basic medicine
lcsh:QH426-470
chicken
Mutant
Single-nucleotide polymorphism
Genome-wide association study
Biology
Carbohydrate metabolism
Polymorphism, Single Nucleotide
Article
03 medical and health sciences
chemistry.chemical_compound
genetic basis
0302 clinical medicine
Increased muscle glycogen content
INDEL Mutation
Genetics
Animals
Humans
Indel
Muscle, Skeletal
Gene
Genetics (clinical)
Adaptor Proteins, Signal Transducing
genome-wide association study
Glycogen
Calcium-Binding Proteins
Molecular Sequence Annotation
Introns
muscle glycogen
lcsh:Genetics
030104 developmental biology
Phenotype
chemistry
variation
Chickens
030217 neurology & neurosurgery
- Language
- English
- ISSN
- 2073-4425
Glucose metabolism plays an important role in many normal and pathological physiological processes in the body. The breakdown and synthesis of muscle glycogen provides ATP for muscle activities. A genome-wide association study for muscle glycogen was performed in 473 Jingxing yellow chickens to identify significant single nucleotide polymorphisms (SNPs) and insertions and deletions (INDELs) involved in muscle glycogen metabolism. A total of nine SNPs (p <
1/699341) and three INDELs (p <
1/755733) reached a significant level of potential association. The following results were obtained through a series of analyses, including additive effects and gene function annotation. Two significant SNPs were found in introns 12 and 13 of copine 4 (CPNE4) on chromosome 2. The wild-type and mutant individuals had significant differences in glycogen metabolism at two loci (p <
0.01 for both). Individuals carrying two mutations had increased muscle glycogen content. According to the gene annotation of chromosome 11, there is a significant INDEL in intron 6 of naked cuticle homolog 1 (NKD1). After the INDEL mutation, the glycogen content increased significantly. There was a significant difference between wild-type and mutant individuals (p <
0.01). These mutations likely affecting two genes (CPNE4 and NKD1) may affect glycogen storage in a pleiotropic manner. Gene annotation indicates that CPNE4 and NKD1 may affect the process of glucose metabolism. Our findings contribute to understanding the genetic regulation of muscle glycogen metabolism and provide theoretical support.