DNA methylation regulates exercise-induced changes in the skeletal muscle transcriptome. However, the specificity and time-course responses in the myogenic regulatory factors DNA methylation and mRNA expression following divergent exercise modes is unknown.To compare the time-course changes in DNA methylation and mRNA expression for selected myogenic regulatory factors (MYOD1, MYF5, and MYF6) immediately post-, 4 and 8 h following a single bout of resistance (RE), high-intensity interval (HIIE), and concurrent exercise (CE).Nine healthy, but untrained males (age 23.9 ± 2.8y, body mass 70.1 ± 14.9 kg, peak oxygen uptake [VO2peak] 41.4 ± 5.2 ml·kg-1·min-1, mean ± SD) performed a counter-balanced, randomized order of RE (4x8-12 repetition maximum), HIIE (12x1 min sprints at VO2peak running velocity) and CE (RE followed by HIIE). Skeletal muscle biopsies (v. lateralis) were taken before (REST) immediately (0 h), 4 and 8 h after each exercise bout.Compared to REST, MYOD1, MYF5, and MYF6 mean methylation across all CpGs analysed was reduced after 4 and 8 h in response to all exercise protocols (P0.05). Reduced levels of MYOD1 methylation were observed after HIIE and CE compared to RE (P0.05). Compared to REST, all exercise bouts increased mRNA expression over time (MYOD1 at 4 and 8 h, and MYF6 at 4 h; P0.05). MYF5 mRNA expression was lower after 4 h compared to 0 h and higher at 8 h compared to 4 h (P0.05).We observed an interrelated but not time-aligned response between the exercise-induced changes in MRFs demethylation and mRNA expression following divergent exercise modes. Despite divergent contractile stimuli, changes in DNA methylation and mRNA expression in skeletal muscle were largely confined to the late (4-8 h) recovery period and similar between the different exercise challenges.