Additional file 1: Table S1. Germacrene D synthases from different organisms. Table S2. Strains and plasmids used in this study. Table S3. Primers used in this study. Table S4. Terpene synthases used for phylogenetic analysis. Table S5. The specific rotation of the product of AcTPS1 and (–)-Germacrene D standard. Table S6. The germacrene D production in different engineered yeaststrains. Fig. S1. Amino acids alignment of the sesquiterpene synthases from Acremonium chrysogenum. Fig. S2. Amino acids alignment of STC1, AcTPS1 and AcTPS3. Fig. S3. The transcription of Actps1to Actps5 inA. chrysogenum. Fig. S4. GC spectrum and the corresponding Mass spectra of sesquiterpenes biosynthesized by AcTPS5. Fig. S5. GC spectrum and the corresponding Mass spectra of sesquiterpenes biosynthesized by AcTPS2. Fig. S6. GC spectrum and the corresponding Mass spectra of sesquiterpenes biosynthesized by AcTPS3. Fig. S7. (–)-Germacrene D standard mass spectrum detected by GC-MS. Fig. S8. Purified AcTPS1 protein from recombinant Escherichia coli. Fig. S9. The mass spectra of the AcTPS1 enzymatic product. Fig. S10. 13C NMR spectra of (–)-Germacrene D (400 MHz, CDCl3).Fig. S11. 1H NMR spectrum of (–)-Germacrene D 30 (400 MHz, CDCl3). Fig. S12. The schematic of gene editing with CRISPR/Cas9 system mediated by a recyclable gRNA plasmid. Fig. S13. GC spectrum of SC1-SC12. Fig. S14. GC spectrum of SC13-SC21, JCR27 and germacrene D standard.