Miniaturization of immunoassay by using a novel module-level immunosensor with polyaniline-modified nanoprobes that incorporate impedance sensing and paper-based sampling
- Resource Type
- Authors
- Da-Huei Lee; Hsun-Pei Wu; Yao-Wei Huang; Shih-Min Chen; Cheng-Hsin Chuang; Yuan-Chu Yu; Ting-Feng Wu; Cheng-Ho Chen
- Source
- Microfluidics and Nanofluidics. 16:869-877
- Subject
- Materials science
medicine.diagnostic_test
Nanotechnology
Multielectrode array
Dielectrophoresis
Condensed Matter Physics
Flexible electronics
Electronic, Optical and Magnetic Materials
Immunoassay
LCR meter
Electrode
Materials Chemistry
medicine
Miniaturization
Electrical impedance
Biomedical engineering
- Language
- ISSN
- 1613-4990
1613-4982
This work presents a module-level impedance measurement system integrated with a disposable immunosensor for the immunoassay of bladder cancer cell lysate (T24) to a specific antibody (galectin-1). The immunosensor consisted of a flexible printed circuit patterned with an interdigital microelectrode array which immobilized polyaniline-modified nanoprobes on an electrode surface by dielectrophoresis. A quantitative sampling of cell lysate without a pump was made by using paper as the cell lysate carrier and sweeping a moistened paper over the sensing area of interdigital microelectrode array for sampling. In this study, the impedance measurement results of the module-level system were compared with those measured by the precision LCR meter, in which the error is