Effects of lipid loading on the hypoxic and post-hypoxic GBM cell secretome. A and B, Maintained effect of lipid loading on protein secretion in reoxygenated, post-hypoxic conditions: GBM cells (U-87 MG) were grown in hypoxia without (LD−) and with LDL (LD+) and then reoxygenated for 6 h (A) and 48 h (B). Shown is linear fold change (FC) of protein levels in LD+ vs LD− cells as determined by proximity extension assay. C and D, ELISA quantification of VEGF-A (C) and HGF (D) in the secretome of GBM cells grown in normoxia (N), hypoxia without LDL (H) or in hypoxia with LDL at the indicated concentrations. LDL = LDL only (50 μg/ml). # = Not detected. (PDF 16 kb)