Introduction The necessity of studying the peculiarities of the structural organization of the testes is determined by its participation in performing important functions for the body – making spermatozoa and the production of male sex hormones. Despite the presence of works devoted to the study of the peculiarities of the structural organization of the testes, many questions remain unresolved or require clarification. Materials and methods As a biological test object used white Mature rats male Wistar rats at the age of 2 months. Seminal glands of male rats were used as a trial material for study. Tissue samples was investigated using a digital microscope Axio Imager.M2 (ZEISS, Japan) with software for image analysis AxioVision SE64 Rel. 4.8.3 and ZEN 2011. When sightseeing microscopy studied morphological features of the structure of the testes, and then determined their morphometric parameters. Results Found that in rats-males aged 2 months in the testes, the proportion of interstitial tissue to the area of convoluted seminiferous tubules is about 1:30. It is shown that in the period of puberty of male white rats the highest percentage of the total amount of the spermatogenic cells are Mature male sex cells – spermatozoa. Second in quantitative terms are male stem cells spermatogonia. Discussion Development and sexual differentiation of the testes is a complex process. The most important indicator of the structural and functional development of the testis is characteristic of the spermatogenic reservoir. The obtained results clearly demonstrate the maturity of experimental animals.