Expression of NGN3 and hairy and enhancer of split-1 (HES1) mRNA following treatment with AKT inhibitor API-1. Quantitative RTPCR results of four biological replicate samples (A–D) treated with API-1 for four days. RQ, relative quantification of NGN3 and HES1 normalized to the level of cyclophillin A. ΔCt, change in threshold cycle (Ct). Mean and standard error of the mean (SEM), results of Student’s t-test (TTEST) and percentage of control are shown. (DOCX 49 kb)